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normal human prostate epithelial cells pwr 1e  (ATCC)


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    ATCC normal human prostate epithelial cells pwr 1e
    Normal Human Prostate Epithelial Cells Pwr 1e, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human prostate epithelial cells pwr 1e/product/ATCC
    Average 93 stars, based on 25 article reviews
    normal human prostate epithelial cells pwr 1e - by Bioz Stars, 2026-05
    93/100 stars

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    93
    ATCC normal human prostate epithelial cells pwr 1e
    Normal Human Prostate Epithelial Cells Pwr 1e, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human prostate epithelial cells pwr 1e/product/ATCC
    Average 93 stars, based on 1 article reviews
    normal human prostate epithelial cells pwr 1e - by Bioz Stars, 2026-05
    93/100 stars
      Buy from Supplier

    93
    ATCC human prostate normal epithelial pwr1e cells
    Increased expression of HA promotes transformation of normal epithelial cells by augmenting expression and activation of the AR gene and soft agar colony formation. A–C , increased HA synthesis transforms <t>PWR1E</t> cells to be non-responsive to DT (10 ng/ml). HA and PSA in the medium were measured by an ELISA-like method ( A ) and the ELISA method ( B ), and colony formation in soft agar was detected by tetrazolium dye ( C ). The data in these experiments are representative of three experiments and, where indicated, are the mean of three experiments ± S.D. ( error bars ).
    Human Prostate Normal Epithelial Pwr1e Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate normal epithelial pwr1e cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    human prostate normal epithelial pwr1e cells - by Bioz Stars, 2026-05
    93/100 stars
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    Increased expression of HA promotes transformation of normal epithelial cells by augmenting expression and activation of the AR gene and soft agar colony formation. A–C , increased HA synthesis transforms PWR1E cells to be non-responsive to DT (10 ng/ml). HA and PSA in the medium were measured by an ELISA-like method ( A ) and the ELISA method ( B ), and colony formation in soft agar was detected by tetrazolium dye ( C ). The data in these experiments are representative of three experiments and, where indicated, are the mean of three experiments ± S.D. ( error bars ).

    Journal: The Journal of Biological Chemistry

    Article Title: Stromal Hyaluronan Interaction with Epithelial CD44 Variants Promotes Prostate Cancer Invasiveness by Augmenting Expression and Function of Hepatocyte Growth Factor and Androgen Receptor *

    doi: 10.1074/jbc.M110.104273

    Figure Lengend Snippet: Increased expression of HA promotes transformation of normal epithelial cells by augmenting expression and activation of the AR gene and soft agar colony formation. A–C , increased HA synthesis transforms PWR1E cells to be non-responsive to DT (10 ng/ml). HA and PSA in the medium were measured by an ELISA-like method ( A ) and the ELISA method ( B ), and colony formation in soft agar was detected by tetrazolium dye ( C ). The data in these experiments are representative of three experiments and, where indicated, are the mean of three experiments ± S.D. ( error bars ).

    Article Snippet: Human prostate normal epithelial PWR1E cells , DT-dependent transformed human prostate epithelial LNCaP cells, and prostate cancer-associated myofibroblast cells (from ATCC, Manassas, VA) were maintained in their respective media.

    Techniques: Expressing, Transformation Assay, Activation Assay, Enzyme-linked Immunosorbent Assay

    Increased HA stimulates CD44v9 expression, and silencing CD44v9 inhibits expression of AR and activation of cMet and IGF1R. Empty vector transfectant ( lane 1 ) and untreated PWR1E-HAS2 ( lane 2 ) were controls. PWR1E-HAS2 stable transfectants were transfected with control siRNA ( lane 3 ), control shRNA ( lane 4 ), CD44v9 siRNA ( lane 5 ), or pSicoRCD44v9 shRNA ( lane 6 ) plasmids (0.5 μg/5 × 10 5 cells). Expressions of proteins and message levels were determined by Western blots ( WB ) from the lysates and mRNA by RT-PCR from the total RNA of the transfectants. The data in these experiments are representative of three experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Stromal Hyaluronan Interaction with Epithelial CD44 Variants Promotes Prostate Cancer Invasiveness by Augmenting Expression and Function of Hepatocyte Growth Factor and Androgen Receptor *

    doi: 10.1074/jbc.M110.104273

    Figure Lengend Snippet: Increased HA stimulates CD44v9 expression, and silencing CD44v9 inhibits expression of AR and activation of cMet and IGF1R. Empty vector transfectant ( lane 1 ) and untreated PWR1E-HAS2 ( lane 2 ) were controls. PWR1E-HAS2 stable transfectants were transfected with control siRNA ( lane 3 ), control shRNA ( lane 4 ), CD44v9 siRNA ( lane 5 ), or pSicoRCD44v9 shRNA ( lane 6 ) plasmids (0.5 μg/5 × 10 5 cells). Expressions of proteins and message levels were determined by Western blots ( WB ) from the lysates and mRNA by RT-PCR from the total RNA of the transfectants. The data in these experiments are representative of three experiments.

    Article Snippet: Human prostate normal epithelial PWR1E cells , DT-dependent transformed human prostate epithelial LNCaP cells, and prostate cancer-associated myofibroblast cells (from ATCC, Manassas, VA) were maintained in their respective media.

    Techniques: Expressing, Activation Assay, Plasmid Preparation, Transfection, Control, shRNA, Western Blot, Reverse Transcription Polymerase Chain Reaction