Journal: The Journal of Biological Chemistry
Article Title: Stromal Hyaluronan Interaction with Epithelial CD44 Variants Promotes Prostate Cancer Invasiveness by Augmenting Expression and Function of Hepatocyte Growth Factor and Androgen Receptor *
doi: 10.1074/jbc.M110.104273
Figure Lengend Snippet: Increased HA stimulates CD44v9 expression, and silencing CD44v9 inhibits expression of AR and activation of cMet and IGF1R. Empty vector transfectant ( lane 1 ) and untreated PWR1E-HAS2 ( lane 2 ) were controls. PWR1E-HAS2 stable transfectants were transfected with control siRNA ( lane 3 ), control shRNA ( lane 4 ), CD44v9 siRNA ( lane 5 ), or pSicoRCD44v9 shRNA ( lane 6 ) plasmids (0.5 μg/5 × 10 5 cells). Expressions of proteins and message levels were determined by Western blots ( WB ) from the lysates and mRNA by RT-PCR from the total RNA of the transfectants. The data in these experiments are representative of three experiments.
Article Snippet: Human prostate normal epithelial PWR1E cells , DT-dependent transformed human prostate epithelial LNCaP cells, and prostate cancer-associated myofibroblast cells (from ATCC, Manassas, VA) were maintained in their respective media.
Techniques: Expressing, Activation Assay, Plasmid Preparation, Transfection, Control, shRNA, Western Blot, Reverse Transcription Polymerase Chain Reaction